Composite

Part:BBa_K2055016:Design

Designed by: Raul Garza & Carlos Vasquez   Group: iGEM16_Tec-Monterrey   (2016-09-28)


Part designed for verification and characterization of the FUR/LacI inverter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 204
    Illegal XhoI site found at 389
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 344
    Illegal AgeI site found at 2394
    Illegal AgeI site found at 2506
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 125
    Illegal BsaI.rc site found at 341


Design Notes

LacI and FUR proteins are optimized for Acidithiobacillus ferrooxidans. It has two RBS after the constitutive promoter and before the FUR CDS.


Source

Composite part

References